Elisa Kit

IgG is the main component of immunoglobulins in serum, with a total content of approximately 75% -80%, of which 40-50% is distributed in serum and the rest in tissues.
The half-life of IgG is about 20-23 days, and it is the main antibody for re immune response, usually a high affinity antibody.
IgG is mainly synthesized and secreted by plasma cells in the spleen and lymph nodes, mainly in monomeric form, with a molecular weight of approximately 150KD.
The IgG of normal individuals includes four subtypes, with IgG1 accounting for 60-70%, IgG2 accounting for 15-20%, IgG3 accounting for 5-10%, and IgG4 accounting for 1-7%.

This experiment used double antibody sandwich Elisa。
Pre coat the enzyme-linked immunosorbent assay (Elisa) plate with anti-mouse IgG total monoclonal antibody,
Add moderately diluted samples and standards, including IgG Total will bind to its monoclonal antibody and wash away free components;
Add enzyme-linked antibodies, enzyme-linked antibodies and mouse IgG bound to monoclonal antibodies
Total binding forms an immune complex, washing away unbound enzyme-linked antibodies;
Add chromogenic agent, if there is IgG in the reaction well. In total, horseradish peroxidase can turn colorless colorants blue;
The termination liquid turns yellow.
The OD value is measured at 450nm, and the concentration of IgG total is directly proportional to the OD450 value.
The concentration of IgG total in the sample can be calculated by plotting a standard curve.

The unopened reagent kit is stored at 2-8 ºC for a validity period of 6 months. It is recommended to use it within one month after opening.
After receiving the product, the standard sample is stored at -20 ºC, while other components are stored at 2-8 °C

1. The reagent kit should be stored at 2-8 ℃. Except for the standard solution after re dissolution, other components cannot be frozen.
2. The concentration of enzyme-linked immunosorbent antibodies is extremely low, and transportation bumps and possible inversion can cause the liquid to adhere to the tube wall or bottle cap. Please centrifuge before use to allow the liquid attached to the tube wall or bottle cap to settle at the bottom of the tube.
3. Different batch numbers of reagents cannot be mixed.
4. To avoid cross contamination, please use a disposable suction head.
5. Termination liquids and colorants are corrosive and should be avoided from direct contact with the skin and mucous membranes. Once in contact with these liquids, please rinse with plenty of water as soon as possible.
6. Prepare the washing solution using a clean plastic container and thoroughly mix the various components and samples in the reagent box before use.
7. When washing the enzyme-linked immunosorbent assay (ELISA) plate, it should be thoroughly dried and the absorbent paper should not be directly placed into the ELISA reaction well to absorb water
8. Do not mix or replace the components of this product with reagents from other sources. Components of reagent kits with different batch numbers cannot be mixed. Please use this product within its expiration date
9. It is recommended to use double or triple wells for standard and sample testing in the experiment, and the order of adding reagents should be consistent to ensure that all reaction wells are incubated for the same time
10. Adequate mixing is particularly important for the reaction results, and it is best to use a micro oscillator (using the lowest frequency for oscillation)
11. Avoid drying the enzyme-linked immunosorbent assay (ELISA) plate during operation, as drying can quickly inactivate the biological components on the ELISA plate and affect the experimental results
12. Dilute the sample appropriately to ensure that the sample values fall within the range of the standard curve, depending on the different levels of high, medium, and low factors to be tested,
Suggest diluting the sample at 1:100, 1:10, and 1:2. If the OD value of the sample is higher than the highest standard, increase the dilution appropriately and repeat the test
13. Differences in standard diluents, operators, pipetting methods, washing methods, incubation time and temperature, and batches of reagent kits may all lead to differences in results
14. This method can effectively eliminate interference from soluble receptors, binding proteins, and other factors in biological samples.